ABSTRACT : The paper describes a field survey performed to determine the prevalence of hydatid cyst disease in cattle at
slaughters in Al-Diwanyah abattoir. The study lasted for five months from November 2017 to April 2018. During the study, 200
cattle were examined. The study included 15 samples were collected from fluids and the germinal layer. The isolation and
identification of E. granulosus were done using microscopic visualization followed by confirmation using a polymerase chain
reaction (PCR) technique targeting the antigen B (AgB2) gene. The results indicate that(7.5%) of cattle were infected. The
livers of cattle demonstrated a higher incidence of hydatid cysts than the lungs, with 6.66% oflivers and 20% of lung being
infected, respectively. Also, the results of the study showed that the sex of the slaughtered animals has non-significant effect on
the distribution of HC, as the rate of HC in males and females were close. The microscopic results revealed the presence of
characteristic cysts. All E. granulosus isolates in cows appeared to be contained this gene show one distinct band (MW400 bp)
when electrophoresed on agarose gel. The results of this study indicated that the PCR technique had a high specify in the
detection of E. granulosus especially this species that encoded to AgB2 gene isolated from cowsin comparison to other routine
diagnostic tests. … قراءة المزيد